Regular Sequencing
- Normal Sequencing
- Difficult Sequencing
- Additional Services
Special Sequencing
- Microbial Identification
- Full Sequencing
- Viral genome sequencing
- Mitochondrial DNA sequencing
- Bisulfite sequencing
- Fragment Analysis
- Cell line authentication
- NGS Integration service
- Designed PCR Sequencing (DPS)
Universal Primer list
Vector-Binding Primer
Troubleshooting
Universal Primer list
Vector primer
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Primer Sequence Primer Sequence T7 TAA TAC GAC TCA CTA TAG GG MATCHMAKER5 GAA GAT ACC CCA CCA AAC T7terminator GCT AGT TAT TGC TCA GCG G MATCHMAKER3 GTG AAC TTG CGG GGT TTT TCA GTA TCT ACG AT T3 ATT AAC CCT CAC TAA AG GAL4(-BD)-3 TTT TCG TTT TAA AAC CTA AGA GT SP6 ATT TAG GTG ACA CTA TAG GAL4(-BD)-5 TCA TCG GAA GAG AGT AGT BGH-R CTA GAA GGC ACA GTC GAG GC pJET1.2-F CGA CTC ACT ATA GGG AGA GCG GC M13F(-40) GTT TTC CCA GTC ACG AC pJET1.2-R AAG AAC ATC GAT TTT CCA TGG CAG M13R(-40) CAG GAA ACA GCT ATG AC pET-upstream ATG CGT CCG GCG TAG AGG M13F(-20) GTA AAA CGA CGG CCA GT DuetUP2 TTG TAC ACG GCC GCA TAA TC M13R(-20) GCG GAT AAC AAT TTC ACA CAG DuetDown1 GAT TAT GCG GCC GTG TAC AA pGEX5 GGG CTG GCA AGC CAC GTT TGG TG ACYCDuetUP1 GGA TCT CGA CGC TCT CCC T pGEX3 CCG GGA GCT GCA TGT GTC AGA GG pFB-F GGA TTA TTC ATA CCG TCC CA CMV-F CGC AAA TGG GCG GTA GGC GTG pFB-R CAA ATG TGG TAT GGC TGA TT CMV30 AAT GTC GTA ATA ACC CCG CCC CGT TGA CGC pMAL-F AAC ATC CCG CAG ATG TCC CMV24 TAT TAG GAC AAG GCT GGT GGG CAC pMAL-R CAA GCT GCC ATT CGC CAT EGFP-C CAT GGT CCT GCT GGA GTT CGT G pBad-F ATG CCA TAG CAT TTT TAT CC EGFP-N CGT CGC CGT CCA GCT CGA CCA G pBad-R GAT TTA ATC TGT ATC AGG SV40 pAR GAA ATT TGT GAT GCT ATT GC pQE-forward CCC GAA AAG TGC CAC CTG RV3 CTA GCA AAA TAG GCT GTC CC pQE-reverse GTT CTG AGG TCA TTA CTG G RV4 GAC GAT AGT CAT GCC CCG CG U6(FLAP) CAG TGC AGG GGA AAG AAT AGT AGA C GL1 TGT ATC TTA TGG TAC TGT AAC TG U6pro GGG CAG GAA GAG GGC CTA T GL2 CTT TAT GTT TTT GGC GTC TTC CA EBV-R GTG GTT TGT CCA AAC TCA TC mCherry-N GTG GCC GTT CAC GGA GCC IRES-F CTG AAG GAT GCC CAG AAG mCherry-C CGC CTA CAA CGT CAA CAT CA IRES-R GAC GGC AAT ATG GTG GAA 96gIII CCC TCA TAG TTA GCG TAA CG
Microbial Identification primer
- Bacteria
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Target region Primer Sequence 16s rRNA 27F AGA GTT TGA TCM TGG CTC AG 337F GAC TCC TAC GGG AGG CWG CAG 518F CCA GCA GCC GCG GTA ATA CG 785F GGA TTA GAT ACC CTG GTA 1100F YAA CGA GCG CAA CCC 518R ATT ACC GCG GCT GCT GG 800R TAC CAG GGT ATC TAA TCC 907R CCG TCA ATT CMT TTR AGT TT 1100R GGG TTG CGC TCG TTG 1492R GGT TAC CTT GTT ACG ACT TC 23s rRNA 1f TCC GAA TGG GGA AAC CC 16r GGA ACT TAC CCG ACA AGG 9f AAG AGG GAA ACA RCC CAG A 23r TTC GTG CTT AGA TGC YTT CA - Fungi / Yeast
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Target region Primer Sequence 5.8s rRNA(ITS) ITS1 TCC GTA GGT GAA CCT GCG G ITS4 TCC TCC GCT TAT TGA TAT GC 18s rRNA NS1 GTA GTC ATA TGC TTG TCT C NS8 TCC GCA GGT TCA CCT AC GGA 25~28s rRNA
(D1/D2 domain)NL1 GCA TAT CAA TAA GCG GAG GAA AAG NL4 GGT CCG TGT TTC AAG ACG G 25~28s rRNA
(D1/D2/D3 domain)LROR ACC CGC TGA ACT TAA GC LR7 TAC TAC CAC CAA GAT CT
