Sanger sequencing

Regular Sequencing

  • Normal Sequencing
  • Difficult Sequencing
  • Additional Services

Special Sequencing

  • Microbial Identification
  • Full Sequencing
  • Viral genome sequencing
  • Mitochondrial DNA sequencing
  • Bisulfite sequencing
  • Fragment Analysis
  • Cell line authentication
  • NGS Integration service
  • Designed PCR Sequencing (DPS)

Universal Primer list

Vector-Binding Primer

Troubleshooting

주문

일반문의

Normal Sequencing

Plasmid나 PCR product를 지정하신 primer를 이용하여 염기서열 분석을 수행하는 서비스입니다.

샘플 준비

Template

  • Plasmid : Concentration 150-200 ng/µl, Volume 15 µl
  • PCR product (purified) : Concentration 50 ng/µl, Volume 15 µl
  • PCR product (non-purified) : Concentration 50 ng/µl, Volume 20 µl

Primer

  • Concentration : 5 pmole/㎕ , Volume : 10 µl
  • Tm : 50~55 ℃, GC content : 40~60%