코스모진텍 서비스

  • Bacterial Expression

해외 서비스

  • Bacterial Expression
  • Mammalian Cell Expression
  • Baculovirus Expression
  • Yeast Expression

주문

일반문의

Mammalian Cell Expression

Mammalian cell에서의 단백질 발현은 다른 진핵생물 발현 시스템과 비교하여 glycosylation의 종류와 방식이 native protein과 유사하고 multi-subunit protein에 정확하게 조립될 수 있습니다.

서비스 장점

  • 자체 Endotoxin-free
  • 효율적인 단백질 folding 및 분비 발현
  • 분자적 구조, 물리적 화학적 특성 및 생물학적 기능에서 native protein과 유사
  • optimization 이후 높은 수율
  • 다양한 cell lines과 발현 방법 : 높은 성공률과 발현률 증가

활용 분야

  • Complex higher eukaryotes proteins
  • Purified protein (structure, enzymology, drug discovery)
  • Protein therapeutics
  • Cell-based studies

Service Process

Plasmid

STEP 01 Plasmid Construction
플라스미드 구축
Process
  • Codon optimization;gene synthesis
  • The PCR amplification products are ligated to the vectors e.g. pSec series, pCMV series and pcDNA series vectors
  • Transform TOP10 E.coli competent cells
  • Obtain the correct recombinant plasmid
Cusabio Features
Multiple vectors optimization, More options for customers

In order to improve the success rate of expression and achieve higher yield, in addition to conventional N-terminal fusion protein expression, we also provide C-terminal fusion protein, which retains the bioactivity of the protein while ensuring high purity.



STEP 02 Small scale expression
소규모 발현
Process
  • Prepare the transfection grade recombinant plasmid in large quantities
  • Transient transfect HEK293, CHO and other cells
  • Detect expression products
Cusabio Features
Optimization of transfection conditions

Set different transfection conditions, select the optimal experimental conditions according to the test results.




STEP 03 Small scale expression
배양 세포 scale-up
Process
  • Scale up the culture cells and transfect
  • Explore different chromatographic conditions including ion exchange, hydrophobic and others by using AKTA, and then determine the optimal purification method.
Cusabio Features
Multi-condition expression scheme

According to the protein localization and the best experimental conditions in the small test expression, select different cell lines and different ways of transfection, which can increase the expression quantity, greatly improve the protein expression.




STEP 04 Additional Services
특수 가공 Optional
Process
  • ChargedTag removal by restriction digestion
  • Free Filter-sterilization
  • Free Endotoxin removal
  • Free Lyophilization ※ Note : Lyophilization and Filter-sterilization cannot be applied simultaneously
Cusabio Features
Flexible additional services

Customers can flexibly choose from a variety of additional services to their specific needs, e.g. Endotoxin removal, Filter-sterilization, Tag removal, Lyophilization, etc. Some are complimentary, and some require additional charge.




STEP 05 Quality Control
품질 검증
Process
  • Testing of purity, concentration, etc.
  • QC report is provided
Cusabio Features
Detailed COA report

Detailed product data sheet and COA are provided for each project.