코스모진텍 서비스

  • Bacterial Expression

해외 서비스

  • Bacterial Expression
  • Mammalian Cell Expression
  • Baculovirus Expression
  • Yeast Expression

주문

일반문의

Baculovirus Expression

안전성이 높은 진핵생물 발현 시스템입니다. 높은 분자량의 단백질을 동시에 발현시킬 수 있으며 단백질 발현 후 가공과정을 통하여 원래의 형태와 유사한 단백질을 얻을 수 있습니다.
Transfer vector, baculovirus vector 및 host cell로 구성되며 바이러스 백신 개발 (ex - 인플루엔자 백신, HPV 백신, 세포 신호 전달 단백질 및 cytokine, kinase 개발) 등에 사용됩니다.

서비스 장점

  • Large gene fragment 운반 능력으로 large protein 발현 가능
  • 높은 안전성 : Baculovirus는 엄격한 종 특이성으로 활성이 제한적
  • Post-translational modification을 통해 높은 생물활성을 가진 단백질을 효율적으로 발현
  • 증폭이 쉽고 대규모의 recombinant protein을 생산할 수 있음
  • Unique bacmid based expression system : 기존 방식보다 낮은 가격, 대용량 suspension transfection, 높은 titer

활용 분야

  • Purified protein (structure, enzymology, drug discovery)
  • 바이러스 백신 개발
  • Cell signaling protein
  • Cytokine, Kinase, etc.

Service Process

  • Steps Project Process Cusabio Features
    1 Plasmid
    Plasmid construction     		Codon optimization; gene synthesis Vector optimization

    In order to improve the success rate of expression and achieve higher yield, we provide protein expression using C-terminal fusion tags in addition to conventional N-terminal tags, which retains the bioactivity of the protein while ensuring high purity.
    The PCR product is ligated to the expression vectors e.g. pFastbac1-KHM, pFastBac1-MBP, etc.
    Transform TOP10 E.coil competent cells
    Obtain the correct recombinant plasmid
    2
    Preparation of recombinant Bacmid and high titer virus     		Transform DH10Bac cells to get recombinant Bacmid; PCR analysis; Isolate recombinant bacmid DNA Suspension transfection

    Unique suspension transfection method greatly increases the protein expression level, and effectively shortens the experimental cycle.
    Transfect recombinant Bacmid DNA into insect cells to obtain baculovirus, and detect expression level by SDS-PAGE; Repeat the infection if necessary
    3
    Scale up expression and purification     		Infect insect cells with appropriate baculovirus Expression optimization

    After optimization, the large amount of protein can be obtained by infecting host cells with low-passage virus.
    The target protein is purified by affinity chromatography, ion exchange, hydrophobic and molecular sieves.
    4
    Additional services (Optional)     		Charge Tag removal by restriction digestion Flexible additional services

    Customers can flexibly choose from a variety of additional services to their specific needs, e.g. Endotoxin removal, Filter-sterilization, Tag removal, Lyophilization, etc. Some are complimentary, and some require additional charge.
    Free Filter-sterilization;
    Endotoxin removal;
    Lyophilization
    (Note: Lyophilization and Filter-sterilization can not be met simultaneously)
    5
    Quality Control     		Testing of purity, concentration, etc.
    QC report is provided.    		 Detailed COA report

    Detailed product data sheet and COA are provided for each project.