- Applications
- - Hot-start PCR
- - Genotyping
- - Real-time PCR
- - Multiplex PCR
Supplied reagents
LaboPass™ IPh-Taq DNA Polymerase is provided with optimized buffer to improved PCR yield.
- 5X IPh-Taq Reaction Mix
- The 5X IPh-Taq Reaction Mix contains buffers, dNTPs, Mg2+, and glycerol in appropriate concentrations.
- N-Solution
- N-Solution is an additive altering the binding behavior of primer and template and can help the amplification that do not work well under standard PCR condition. we recommend to use the N- Solution only in case of the PCR amplification is not works well or too much non-specific products are observed.
| Component | Volume |
|---|---|
| IPh-Taq polymerase | 250 μl X 2 |
| 5X IPh-Taq Reaction Mix | 1.25 ml X 4 |
| N-Solution | 1 ml X 2 |
PCR performance
LaboPass™ IPh-Taq polymerase was passed from quality control assay for contamination of endo- or exodeoxyribonuclease and the bacterial host DNA.
Comparison of IPh-Taq polymerase with other company’s hot start versions of DNA polymerases. 12 different primer sets designed from human genome were used in this PCR.
PCR reactions were performed using 10 ng of human genomic DNA under the various annealing temperature.
A : IPh-Taq B : Company S[Korea] C : Company T[Japan] D : Company I[Korea]
| Components | Reaction Volumes (μl) | |
|---|---|---|
| 25 | 50 | |
| Template | X μl | X μl |
| Forward Primer (10 pmoles/μl) | 1 μl | 2 μl |
| Reverse Primer (10 pmoles/μl) | 1 μl | 2 μl |
| 5X IPh-Taq Reaction Mix | 5 μl | 10 μl |
| N-Solution [optional] | 0~2.5 μl | 0~5 μl |
| Labopass™ IPh-Taq Polymerase | 0~2.5 μl | 0~5 μl |
| Distilled water | up to 25 μl | up to 50 μl |
High amplification efficiency
Superior activity IPh-Taq polymerase on difficult targets.Different primer sets (ApoE, MD, Prion, COMT) designed from the indicated region of human genome were used in this PCR.
PCRs were performed using 10 ng of human genomic DNA. All of the DNA polymerases are hot-start versions of each brands.
A : Company T[Japan]
B : Company I[Korea]
C : Company B[Korea]
D : Company I[USA]
E : Company S[Korea]
F : IPh-Taq [LaboPass]
Quality control
The PCR amplification efficiency was compared with other commercial Taq polymerase. LaboPass™ IPh-Taq polymerase shows comparable or superior quality.