LaboPass™ Plasmid DNA Purification Kit Mini is designed for rapid isolation of plasmid DNA and provides a fast and convenient tool for an efficient purification of low or high copy plasmid DNA in a comfortable spin format or on a vacuum manifold. This plasmid mini purification kit is highly suitable for routine molecular biology applications.
- High yield and superior purity
- LaboPass™ DNA Purification Kit Mini provides a rapid method for purification of up to 30 μg of plasmid DNA from 1 to 5 ml of bacteria culture. The purified plasmid DNA is ready to use in various molecular biology applications such as transfection, in vitro expression, enzyme digestion, PCR amplification and DNA sequencing.
- Fast and easy-to-use system
- LaboPass™ Plasmid DNA Purification Kit Mini provides a fast and simple plasmid purification method for routine molecular biology applications. This kit does not require DNA phenol extraction or alcohol precipitation and the entire procedure can be completed within 20 minutes.
High Yield
- Compared with other company kits, LaboPass™ Plasmid DNA Purification Kit Mini showed the high yield and purity.
Lane M : 1kb Labo DNA ladderPlasmid DNA : pcDNA3.1
E.coli strain : DH5α
| Supplier | DNA Concentration (ng/µl) | |
|---|---|---|
| Company A | 148.0 | 147.3 |
| Company B | 136.7 | 139.6 |
| Company C | 150.4 | 163.8 |
| LaboPass™ | 292.6 | 330.2 |
Consistent performance
- LaboPass™ Plasmid DNA Purification Kit Mini provides a confidence in results. Especially, Lysis indicator dye is help to success every time.
Plasmid DNAs were prepared from independent culture batch using LaboPass™ Plasmid DNA Purification Kit Mini. Each lane represents the purified plasmid DNA (pcDNA3.1).
Samples were analyzed on 1% agarose gel in TAE buffer.
Lane M : 1 kb Labo DNA ladder
| Concentration(ng/µl) of purified plasmid DNA | |||||||
|---|---|---|---|---|---|---|---|
| 1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 |
| 385.5 | 384.8 | 341.4 | 369.7 | 352.9 | 352.1 | 346.2 | 346.7 |
Superior Purity
Plasmid DNA purified by LaboPass™ Plasmid DNA Purification Kit Mini is ready to use for various molecular biology applications.
- Restriction enzyme digestion
- The purified plasmid DNA by LaboPass™ Plasmid DNA Purification Kit Mini was digested with several kinds of restriction enzyme.
Samples were analyzed on 1% agarose gel in TAE buffer.
- Sequencing analysis
- Automated DNA sequencing was performed on plasmid DNA (pcDNA3.1) purified with LaboPass™ Plasmid DNA Purification Kit Mini.
Sequencer Name : Applied Biosystems, Automatic Sequencer ABI 3730XL Applied
Sequencing System : ABI BigDye® Terminator version 3.1
Lane M : 1kb Labo DNA ladderLane 1 : Purified pcDNA3.1 using LaboPass™ Plasmid DNA Purification Kit Mini
Lane 2 : BamHI digestion of purified pcDNA3.1
Lane 3 : Sall digestion of purified pcDNA3.1
Lane 4 : Double digestion with EcoRI and Sall of purified pcDNA3.1
