* Proteinase K solution 보관 시 주의사항 - 2개월간은 4℃에서 보관, 장기간 보관 시에는 분주하여 -20℃에서 보관 (자세한 사항은 Protocol을 참조해주세요.)
LaboPass™ Tissue Genomic DNA Isolation Kit Mini is optimized for isolation of genomic DNA from various tissue types (e.g. spleen, liver, heart, brain, tail, or insects) or cells and provides a fast and convenient tool for efficient purification of genomic DNA in a comfortable spin format or on a vacuum manifold. This provides reproducible yields of highly purified genomic DNA and allows for the use of the purified genomic DNA in diverse applications.
- High yield and purity
- Genomic DNA can be prepared from 20 mg of tissue or 5 x 106 HEK293 cells using LaboPass™ Tissue Genomic DNA Isolation Kit Mini. The obtained genomic DNA is ready to use in various applications such as PCR, real-time PCR, SNP detection, Southern blotting, sequencing, and cloning.
- Fast and easy-to-use system
- After lysis step, genomic DNA can be obtained within 10 minutes.
High Yield
- Genomic DNA was purified from various mouse tissues (20 mg each) using the LaboPass™ Tissue Genomic DNA Isolation Kit Mini.
The purified genomic DNA was analyzed on 1 % agarose gel in TAE buffer.
Lane M : 1 kb Labo DNA ladder Lane 1 : 400 bp DNA fragment
Lane 2 : 600 bp DNA fragment
Lane 3 : 800 bp DNA fragment
Lane 4 : 1 kb DNA fragment
| Samples | Amounts | Yield | Purity |
|---|---|---|---|
| Lung (Mouse) | 20 mg | 10 - 20 µg | 1.8 - 1.9 |
| Liver (Mouse) | 20 mg | 20 - 40 µg | 1.8 - 1.9 |
| Kidney (Mouse) | 20 mg | 30 - 40 µg | 1.8 - 1.9 |
| Spleen (Mouse) | 20 mg | 20 - 30 µg | 1.8 - 1.9 |
| Brain (Mouse) | 20 mg | 15 - 20 µg | 1.8 - 1.9 |
| Heart (Mouse) | 20 mg | 15 - 20 µg | 1.8 - 1.9 |
| Tail (Mouse) | 20 mg | 10 - 20 µg | 1.8 - 1.9 |
| QBI Cell (Human) | 5 X 106 cells | 15 - 20 µg | 1.8 - 1.9 |
- Compared with other company kits, LaboPass™ Tissue Genomic DNA Isolation Kit Mini showed higher for genomic DNA yields.
Samples were analyzed on 1% agarose gel in TAE buffer.
Lane M : 1 kb Labo DNA ladder
| Samples | Mouse Liver (20mg) | |
|---|---|---|
| DNA concentration (ng/µl) | ||
| Company A | 15.2 | 14.9 |
| Company B | 21.8 | 17.1 |
| Company C | 21.1 | 16.3 |
| Company D | 15.8 | 27.8 |
| LaboPass™ | 30.1 | 29.4 |
Superior Purity
Genomic DNA purified by LaboPass™ Tissue Genomic DNA Isolation Kit Mini is ready to use for various molecular biology applications.
- Restriction enzyme digestion
- Genomic DNA purified by LaboPass™ Tissue Genomic DNA Isolation Kit Mini from various mouse tissues were digested with restriction enzyme. Samples were analyzed on 1% agarose gel in TAE buffer.
- PCR amplification
- PCR amplification was performed with purified genomic DNA using LaboPass™ Tissue Genomic DNA Isolation Kit Mini from various tissue of mouse.
Samples were analyzed on 1% agarose gel in TAE buffer.
Lane M : 1kb Labo DNA ladderLane 1, 3, 5, 7, 9, 11, 13 : Purified genomic DNA from each mouse tissue
Lane 2, 4, 6, 8, 10, 12, 14 : Restriction enzyme digestion (HindIII) of Purified genomic
DNA from each mouse tissue
a : Mouse Actin (700 bp)rb : Mouse GAPDH (400 bp)
Lane M : 1 kb Labo DNA ladder